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TECHNOLOGIES
We develop products and processes for ex vivo gene therapy using lentiviral vectors and CRISPR genome editing technology.
![AV_marquage_CD34_04.jpg](https://static.wixstatic.com/media/663cbb_41280dc3dc55403c83a221dc6ba06646~mv2.jpg/v1/fill/w_210,h_249,al_c,q_80,usm_0.66_1.00_0.01,enc_auto/AV_marquage_CD34_04.jpg)
Cell processing
Cell processing is generally required to prepare cells for ex vivo gene-modification. Cell processing steps include the separation of blood components, magnetic bead cell sorting and cryopreservation.
![Antoine TFF.png](https://static.wixstatic.com/media/663cbb_bb16a7fb3ee94451abd289bbc7054c32~mv2_d_2506_1881_s_2.png/v1/fill/w_210,h_249,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/Antoine%20TFF.png)
Lentiviral Vectors
Advanced-generation recombinant HIV-1-derived lentiviral vectors are used for gene transfer or for genome editing. ART-TG has R&D expertise in LV bioproduction, particularly the development of downstream processes for the purification of LV with various envelope pseudotypes.
![IMG_20190316_172640675 (2).jpg](https://static.wixstatic.com/media/663cbb_a0ba84be82ac41919ec2cb2979bed4b8~mv2_d_3411_1918_s_2.jpg/v1/fill/w_210,h_249,al_c,q_80,usm_0.66_1.00_0.01,enc_auto/IMG_20190316_172640675%20(2).jpg)
CRISPR/Cas
CRISPR Cas9 tools are used for genome editing. Synthetic or vectorized constructs are used and are delivered into cells with various methods including electroporation or infection.
![Image ART-3.png](https://static.wixstatic.com/media/663cbb_175a831e4f9041f29a356cd8723c3eb7~mv2_d_2635_3018_s_4_2.png/v1/fill/w_210,h_250,al_c,q_85,usm_0.66_1.00_0.01,enc_auto/Image%20ART-3.png)
Analytics
Specific quality controls are used to analyze gene-modified cells or materials and include the quantification of genome integrated vector copies, the caracterization of genomic insertion sites, potency assays, infectious and physical titers.